This study was initiated to explore some novel biomarkers like pro-inflammatory markers (chemerin and visfatin) and anti-inflammatory marker (omentin-1) as prognostic factors for cardiovascular complications in type 2 diabetic patients. Forty diabetic patients without cardiovascular disease, 40 diabetic patients with cardiovascular disease and twenty healthy control counterparts were included in this study. Serum chemerin, omentin-1 and visfatin levels were quantified. Receiver operating characteristic curve analysis was done to identify the cut off value for each marker. The mean serum level of chemerin was 57.65?±?15.69&nbsp;ng/l in diabetic patients versus 93.97?±?26.62&nbsp;ng/l for the cardio-diabetic ones (P? less then ?0.0001). The mean serum level of omentin-1 was 8.77?±?1.53&nbsp;ng/ml in diabetic patients versus 1.76?±?0.96&nbsp;ng/ml for the cardio-diabetic ones (P? less then ?0.0001). The mean level of visfatin was 1.44?±?0.71 ug/l in diabetic patients versus 3.92?±?3.32 ug/l for the cardio-diabetic ones (P? less then ?0.0001). Chemerin and Visfatin levels were significantly enhanced in the cardio-diabetic patients with increasing C-reactive protein (CRP), triglycerides (TG), fasting blood glucose (FBG), micro-albumin and cholesterol. https://www.selleckchem.com/products/vorapaxar.html Omentin-1 level was significantly reduced in the cardio-diabetic patients with increasing CRP, TG, FBG, and cholesterol. It was observed that the area under curve for chemerin, omentin-1and visfatin was 0.877, 0.998 and 0.735, respectively. In conclusion, this study evidences that the measuring serum levels of chemerin, omentin-1 and visfatin may help in the prognosis of cardiovascular complications in type 2 diabetic patients. © Association of Clinical Biochemists of India 2018.Abstract The molecular mechanism of iron transfer across placenta in response to maternal anemic status/ iron supplementation is not clear. We hypothesized that maternal iron/ anemia status during early trimesters can be utilized as a biomarker tool to get estimates of placental iron status. Early interventions can be envisaged to maintain optimum placental/ foetal iron levels for healthy pregnancy outcomes. One hundred twenty primigravida were recruited and divided into non-anemic and anemic group on the basis of hemoglobin levels. The groups were randomly allocated to receive daily and weekly iron folic acid (IFA) tablets till six weeks postpartum. Hematological and iron status markers in blood and placenta were studied along with the delivery notes. Weekly IFA supplementation in anemic primigravidas resulted in significantly reduced levels of hematological markers (p less then ?0.01); whereas non-anemic primigravidas showed lower ferritin and iron levels, and higher soluble transferrin receptor levels (p less then ?0.05). At baseline, C-reactive protein and cortisol hormone levels were also significantly lower in non-anemic primigravidas (p less then ?0.05). A significantly decreased placental ferritin expression (p less then ?0.05); and an increased placental transferrin expression was seen in anemic primigravidas supplemented with weekly IFA tablets. A significant positive correlation was observed between serum and placental ferritin expression in anemic pregnant women (r =?0.80; p less then ?0.007). Infant weight, gestational length and placental weight were comparable in both the supplementation groups. To conclude, mother's serum iron / anemia status switches the modulation in placental iron transporter expression for delivering the optimum iron to the foetus for healthy pregnancy outcomes. Trial Registration Clinical Trial Registry-India CTRI/2014/10/005135. © Association of Clinical Biochemists of India 2018.Status of diabetes of an individual is majorly evaluated by the frequent monitoring of glucose estimation. Use of serum samples and inappropriate plasma for estimating glucose is an existing practise in Indian standard of laboratories. There is a strong evidence for&nbsp;occurrence of in vitro glycolysis on the above mentioned specimens. The aim is to study the pre-analytical variations on the glucose estimation of using sodium fluoride-disodium EDTA (NaF-Na2EDTA) plasma (glycolysis inhibiting anticoagulant) and determine the fact behind the activity of glycolysis inhibition on the same. Healthy volunteers 20-35&nbsp;years of both genders consisting of 40 members were selected for the study, and after getting the informed consent form, random blood samples were collected to study the errors of pre-analytical i.e., mixing of NaF-Na2EDTA tube by phlebotomist (no of inversion). Difference in duration from blood collection to centrifugation and a variable in time were taken from centrifugation to analyzing the plasma sample. Comparative studies on EDTA plasma and serum sample were also carried out. The usage of the evacuated blood collection system on NaF-Na2EDTA was shown to have the complete glycolysis inhibitor among all pre-analytical errors, whereas&nbsp;other tubes shown considerable increase in glycolysis. Recently the use of glycolysis inhibitor tubes are come into practise only in accredited or certified laboratories. Hence the authentication of glycolysis inhibition study is mandatory for&nbsp;the pre-analytical variations on the same. © Association of Clinical Biochemists of India 2018.The antioxidant protective effects of gallic acid (GAL) and quercetin (QUE) against oxidative stress induced by di-butyl phthalate (DnBP) in the liver and testis of rats were evaluated in this study. Adult albino Wistar rats (180-225&nbsp;g) were treated with QUE or GAL (50&nbsp;mg/kg) alone or in combination with DnBP (1&nbsp;mL/kg) for 15&nbsp;days. After treatment, tissue samples were taken for determination of glutathione and malondialdehyde levels, and superoxide dismutase and catalase activities. Serial sections of the testis and liver were stained with haematoxylin and eosin for microscopy and seminiferous tubular morphometry. As expected, DnBP induced oxidative stress was evident by increased malondialdehyde level in both organs. Co-treatment with GAL or QUE reversed the malondialdehyde by 45.42, 37.44 and 37.57%, 23.32% and catalase by 52.21, 70.15 and 85%, 38.14% in the testis and liver respectively whereas superoxide dismutase activity and glutathione level were differently modulated parallel to histopathological improvement in both tissues.